| | Definition: | | Mammalian cells are fragile and harder to grow than other cell types, but their large-scale culturing is an economic boon because it allows for the production of proteins that are otherwise difficult/expensive/unethical to extract from living organisms. There are two basic ways of culturing animal cells: - The cells are immobilized on a substrate and then perfused with culture medium;
- The cells are in a free suspension which is very gently mixed and aerated.
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