Meaning of SOUTHERN HYBRIDIZATION

Biology Dictionary
	Definition:		A technique used for searching for a specific DNA fragment. The process is as follows: Separate DNA fragments by gel electrophoresis change pH of gel to basic, thus allowing disruption of H-bonds blot gel with nitrocellulose paper heat paper so as to fix DNA fragments probe with labeled messenger RNA or cDNA wash complementary mRNA/cDNA fragments will have hybridized.
Glossary
	Definition:		A procedure in which DNA restriction fragments are transferred from an agarose gel to a nitrocellulose filter, where the denatured DNA is then hybridized to a radioactive probe (blotting). (See Hybridization)